{"id":8975,"date":"2022-07-20T14:53:46","date_gmt":"2022-07-20T14:53:46","guid":{"rendered":"https:\/\/dev.sequentiabiotech.com\/publication\/fine-tuned-kdm1a-alternative-splicing-regulates-human-cardiomyogenesis-through-an-enzymatic-independent-mechanism\/"},"modified":"2026-02-25T12:07:16","modified_gmt":"2026-02-25T12:07:16","slug":"fine-tuned-kdm1a-alternative-splicing-regulates-human-cardiomyogenesis-through-an-enzymatic-independent-mechanism","status":"publish","type":"publication","link":"https:\/\/www.sequentiabiotech.com\/es\/publication\/fine-tuned-kdm1a-alternative-splicing-regulates-human-cardiomyogenesis-through-an-enzymatic-independent-mechanism\/","title":{"rendered":"Fine-tuned KDM1A alternative splicing regulates human cardiomyogenesis through an enzymatic-independent mechanism"},"content":{"rendered":"<p><strong>Authors:<\/strong> Veronica Astro, Gustavo Ramirez-Calderon, Roberta Pennucci, Jonatan Caroli, Alfonso Saera-Vila, Kelly Cardona- Londo\u00f1o, Chiara Forastieri, Elisabetta Fiacco, Fatima Maksoud, Maryam Alowaysi, Elisa Sogne Andrea Falqui, Federico Gonz\u00e0lez, Nuria Montserrat, Elena Battaglioli, Andrea Mattevi, Antonio Adamo<\/p>\n<p><strong>Institutions<\/strong>:<\/p>\n<ul>\n<li>Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Saudi Arabia<\/li>\n<li>Department of Biology and Biotechnology, University of Pavia, Italy<\/li>\n<li>Sequentia Biotech SL<\/li>\n<li>Department of Medical Biotechnology and Translational Medicine, University of Milan, Italy<\/li>\n<li>Pluripotency for Organ Regeneration, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain<\/li>\n<\/ul>\n<p><strong>Publication<\/strong>: iScience<br \/>\n<strong>Date<\/strong>: July 2022<br \/>\n<strong>Link<\/strong>: <a href=\"https:\/\/www.cell.com\/iscience\/fulltext\/S2589-0042(22)00937-3?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2589004222009373%3Fshowall%3Dtrue\">Fine-tuned KDM1A alternative splicing regulates human cardiomyogenesis through an enzymatic-independent mechanism<\/a><\/p>\n<p><strong>Abstract<\/strong>:<\/p>\n<p>The\u00a0histone\u00a0demethylase\u00a0KDM1A is a multi-faceted regulator of vital developmental processes, including mesodermal and cardiac tube formation during\u00a0gastrulation. However, it is unknown whether the fine-tuning of KDM1A splicing\u00a0isoforms, already shown to regulate neuronal maturation, is crucial for the specification and maintenance of cell identity during cardiogenesis. Here, we discovered a temporal modulation of ubKDM1A and KDM1A+2a during human and mice fetal cardiac development and evaluated their impact on the regulation of cardiac differentiation. We revealed a severely impaired cardiac differentiation in KDM1A<sup>\u2212\/\u2212<\/sup>\u00a0hESCs\u00a0that can be rescued by re-expressing ubKDM1A or catalytically impaired ubKDM1A-K661A, but not by KDM1A+2a or KDM1A+2a-K661A. Conversely, KDM1A+2a<sup>\u2212\/\u2212<\/sup>\u00a0hESCs give rise to functional\u00a0cardiac cells, displaying increased beating amplitude and frequency and enhanced expression of critical\u00a0cardiogenic\u00a0markers. Our findings prove the existence of a divergent scaffolding role of KDM1A splice variants, independent of their\u00a0enzymatic activity, during hESC differentiation into cardiac cells.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"featured_media":9495,"parent":0,"template":"","meta":{"_acf_changed":false},"omics-area":[40],"application-field":[35,37],"solution":[46],"publication-type":[49],"class_list":["post-8975","publication","type-publication","status-publish","has-post-thumbnail","hentry","omics-area-transcriptomics","application-field-basic-research","application-field-health","solution-air","publication-type-peer-reviewed-articles"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.9 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Fine-tuned KDM1A alternative splicing regulates human cardiomyogenesis through an enzymatic-independent mechanism - Sequentia<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sequentiabiotech.com\/es\/publication\/fine-tuned-kdm1a-alternative-splicing-regulates-human-cardiomyogenesis-through-an-enzymatic-independent-mechanism\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Fine-tuned KDM1A alternative splicing regulates human cardiomyogenesis through an enzymatic-independent mechanism - 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