Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis<\/a><\/p>\nAbstract:<\/b><\/p>\n
Whereas stem and progenitor cells proliferate to maintain tissue homeostasis, fully differentiated cells exit the cell cycle. How cell identity and cell-cycle state are coordinated during differentiation is still poorly understood. The\u00a0Drosophila<\/em>\u00a0testis niche supports germline stem cells and somatic cyst stem cells (CySCs). CySCs give rise to post-mitotic cyst cells, providing a tractable model to study the links between stem cell identity and proliferation. We show that, while cell-cycle progression is required for CySC self-renewal, the E2f1\/Dp transcription factor is dispensable for self-renewal but instead must be silenced by the\u00a0Drosophila<\/em>\u00a0retinoblastoma homolog, Rbf, to permit differentiation. Continued E2f1\/Dp activity inhibits the expression of genes important for mitochondrial activity. Furthermore, promoting mitochondrial biogenesis rescues the differentiation of CySCs with ectopic E2f1\/Dp activity but not their cell-cycle exit. In sum, E2f1\/Dp coordinates cell-cycle progression with stem cell identity by regulating the metabolic state of CySCs.<\/p>\n","protected":false},"featured_media":9497,"parent":0,"template":"","meta":{"_acf_changed":false},"omics-area":[40],"application-field":[36,35],"solution":[46],"publication-type":[50],"class_list":["post-8977","publication","type-publication","status-publish","has-post-thumbnail","hentry","omics-area-transcriptomics","application-field-animal-science","application-field-basic-research","solution-air","publication-type-citations"],"acf":[],"yoast_head":"\nCell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis - Sequentia<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/\" \/>\n<meta property=\"og:locale\" content=\"it_IT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis - Sequentia\" \/>\n<meta property=\"og:description\" content=\"Authors: Diego Sainz de la Maza, Silvana Hof-Michel,\u00a0Lee Phillimore, Christian B\u00f6kel, Marc Amoyel Institutions: Department of Cell and Developmental Biology, University College London, UK Department of Developmental Genetics, Philipps University Marburg, Germany Publication: Cell Date: May, 2022 Link: Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/\" \/>\n<meta property=\"og:site_name\" content=\"Sequentia\" \/>\n<meta property=\"article:modified_time\" content=\"2026-02-25T12:06:35+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.sequentiabiotech.com\/wp-content\/uploads\/2025\/06\/45-e1770713816963.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1080\" \/>\n\t<meta property=\"og:image:height\" content=\"750\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Tempo di lettura stimato\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minuti\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/publication\\\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\\\/\",\"url\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/publication\\\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\\\/\",\"name\":\"Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis - Sequentia\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/publication\\\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/publication\\\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/wp-content\\\/uploads\\\/2025\\\/06\\\/45-e1770713816963.png\",\"datePublished\":\"2022-06-10T09:22:39+00:00\",\"dateModified\":\"2026-02-25T12:06:35+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/publication\\\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\\\/#breadcrumb\"},\"inLanguage\":\"it-IT\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/publication\\\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"it-IT\",\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/publication\\\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\\\/#primaryimage\",\"url\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/wp-content\\\/uploads\\\/2025\\\/06\\\/45-e1770713816963.png\",\"contentUrl\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/wp-content\\\/uploads\\\/2025\\\/06\\\/45-e1770713816963.png\",\"width\":1080,\"height\":750},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/publication\\\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/#website\",\"url\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/\",\"name\":\"Sequentia\",\"description\":\"biotech\",\"publisher\":{\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"it-IT\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/#organization\",\"name\":\"Sequentia\",\"url\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"it-IT\",\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/wp-content\\\/uploads\\\/2025\\\/10\\\/Logo_Sequentia_OK.svg\",\"contentUrl\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/wp-content\\\/uploads\\\/2025\\\/10\\\/Logo_Sequentia_OK.svg\",\"width\":662,\"height\":141,\"caption\":\"Sequentia\"},\"image\":{\"@id\":\"https:\\\/\\\/www.sequentiabiotech.com\\\/it\\\/#\\\/schema\\\/logo\\\/image\\\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis - Sequentia","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/","og_locale":"it_IT","og_type":"article","og_title":"Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis - Sequentia","og_description":"Authors: Diego Sainz de la Maza, Silvana Hof-Michel,\u00a0Lee Phillimore, Christian B\u00f6kel, Marc Amoyel Institutions: Department of Cell and Developmental Biology, University College London, UK Department of Developmental Genetics, Philipps University Marburg, Germany Publication: Cell Date: May, 2022 Link: Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis […]","og_url":"https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/","og_site_name":"Sequentia","article_modified_time":"2026-02-25T12:06:35+00:00","og_image":[{"width":1080,"height":750,"url":"https:\/\/www.sequentiabiotech.com\/wp-content\/uploads\/2025\/06\/45-e1770713816963.png","type":"image\/png"}],"twitter_card":"summary_large_image","twitter_misc":{"Tempo di lettura stimato":"2 minuti"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/","url":"https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/","name":"Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis - Sequentia","isPartOf":{"@id":"https:\/\/www.sequentiabiotech.com\/it\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/#primaryimage"},"image":{"@id":"https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/#primaryimage"},"thumbnailUrl":"https:\/\/www.sequentiabiotech.com\/wp-content\/uploads\/2025\/06\/45-e1770713816963.png","datePublished":"2022-06-10T09:22:39+00:00","dateModified":"2026-02-25T12:06:35+00:00","breadcrumb":{"@id":"https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/#breadcrumb"},"inLanguage":"it-IT","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/"]}]},{"@type":"ImageObject","inLanguage":"it-IT","@id":"https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/#primaryimage","url":"https:\/\/www.sequentiabiotech.com\/wp-content\/uploads\/2025\/06\/45-e1770713816963.png","contentUrl":"https:\/\/www.sequentiabiotech.com\/wp-content\/uploads\/2025\/06\/45-e1770713816963.png","width":1080,"height":750},{"@type":"BreadcrumbList","@id":"https:\/\/www.sequentiabiotech.com\/it\/publication\/cell-cycle-exit-and-stem-cell-differentiation-are-coupled-through-regulation-of-mitochondrial-activity-in-the-drosophila-testis\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.sequentiabiotech.com\/it\/"},{"@type":"ListItem","position":2,"name":"Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis"}]},{"@type":"WebSite","@id":"https:\/\/www.sequentiabiotech.com\/it\/#website","url":"https:\/\/www.sequentiabiotech.com\/it\/","name":"Sequentia","description":"biotech","publisher":{"@id":"https:\/\/www.sequentiabiotech.com\/it\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.sequentiabiotech.com\/it\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"it-IT"},{"@type":"Organization","@id":"https:\/\/www.sequentiabiotech.com\/it\/#organization","name":"Sequentia","url":"https:\/\/www.sequentiabiotech.com\/it\/","logo":{"@type":"ImageObject","inLanguage":"it-IT","@id":"https:\/\/www.sequentiabiotech.com\/it\/#\/schema\/logo\/image\/","url":"https:\/\/www.sequentiabiotech.com\/wp-content\/uploads\/2025\/10\/Logo_Sequentia_OK.svg","contentUrl":"https:\/\/www.sequentiabiotech.com\/wp-content\/uploads\/2025\/10\/Logo_Sequentia_OK.svg","width":662,"height":141,"caption":"Sequentia"},"image":{"@id":"https:\/\/www.sequentiabiotech.com\/it\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/www.sequentiabiotech.com\/it\/wp-json\/wp\/v2\/publication\/8977","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sequentiabiotech.com\/it\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/www.sequentiabiotech.com\/it\/wp-json\/wp\/v2\/types\/publication"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.sequentiabiotech.com\/it\/wp-json\/wp\/v2\/media\/9497"}],"wp:attachment":[{"href":"https:\/\/www.sequentiabiotech.com\/it\/wp-json\/wp\/v2\/media?parent=8977"}],"wp:term":[{"taxonomy":"omics-area","embeddable":true,"href":"https:\/\/www.sequentiabiotech.com\/it\/wp-json\/wp\/v2\/omics-area?post=8977"},{"taxonomy":"application-field","embeddable":true,"href":"https:\/\/www.sequentiabiotech.com\/it\/wp-json\/wp\/v2\/application-field?post=8977"},{"taxonomy":"solution","embeddable":true,"href":"https:\/\/www.sequentiabiotech.com\/it\/wp-json\/wp\/v2\/solution?post=8977"},{"taxonomy":"publication-type","embeddable":true,"href":"https:\/\/www.sequentiabiotech.com\/it\/wp-json\/wp\/v2\/publication-type?post=8977"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}