Authors: Valeria Scala, Paola Giorni, Martina Cirlini, Matteo Ludovici, Ivan Visentin, Francesca Cardinale, Anna A. Fabbri, Corrado Fanelli, Massimo Reverberi, Paola Battilani, Gianni Galaverna and Chiara Dall’Asta

Institutions:

  • Department of Environmental Biology, University of Rome “Sapienza”, Rome, Italy
  • Istituto di Entomologia e Patologia Vegetale, Università Cattolica del Sacro Cuore, Piacenza, Italy
  • Food Chemistry and Natural Substances Unit, Department of Organic and Industrial Chemistry, University of Parma, Parma, Italy
  • Department of Agricultural, Food and Forestry Science, University of Turin, Torino, Italy

Publication: Frontiers in Microbiology

Date: December 2014

Full paper: https://www.frontiersin.org/articles/10.3389/fmicb.2014.00669/full

Abstract:

Oxylipins are fatty acid-derived signaling compounds produced by all eukaryotes so far investigated; in mycotoxigenic fungi, they modulate toxin production and interactions with the host plants. Among the many enzymes responsible for oxylipin generation, Linoleate Diol Synthase 1 (LDS1) produces mainly 8-hydroperoxyoctadecenoic acid and subsequently different di-hydroxyoctadecenoic acids. In this study, we inactivated a copy of the putative LDS1 ortholog (acc. N. FVEG_09294.3) of Fusarium verticillioides, with the aim to investigate its influence on the oxylipin profile of the fungus, on its development, secondary metabolism and virulence. LC-MS/MS oxylipin profiling carried out on the selected mutant strain revealed significant quali-quantitative differences for several oxylipins when compared to the WT strain. The Fvlds1-deleted mutant grew better, produced more conidia, synthesized more fumonisins and infected maize cobs faster than the WT strain. We hypothesize that oxylipins may act as regulators of gene expression in the toxigenic plant pathogen F. verticillioides, in turn causing notable changes in its phenotype. These changes could relate to the ability of oxylipins to re-shape the transcriptional profile of F. verticillioides by inducing chromatin modifications and exerting a direct control on the transcription of secondary metabolism in fungi.