Authors: Luming Yang Dal‐Hoe Koo Dawei Li Tao Zhang Jiming Jiang Feishi Luan Susanne S. Renner Elizabeth Hénaff Walter Sanseverino Jordi Garcia‐Mas Josep Casacuberta Douglas A. Senalik Philipp W. Simon Jinfeng Chen Yiqun Weng

Institutions:

  • Horticulture Department, University of Wisconsin, Madison, WI, 53706 USA
  • Department of Biology, University of Munich, 80638 Munich, Germany
  • Centre for Research in Agricultural Genomics Consejo Superior de Investigaciones Científicas‐Institut de Recerca i Tecnologia Agroalimentàries‐Universitat Autònoma de Barcelona‐Universitat de Barcelona, 08193 Barcelona, Spain
  • College of Horticulture, Nanjing Agricultural University, Nanjing, 210095 China

Publication: The Plant Journal

Date: October 2013

Full paper: https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.12355

Abstract:

In the large Cucurbitaceae genus Cucumis, cucumber (C. sativus) is the only species with 2= 2= 14 chromosomes. The majority of the remaining species, including melon (C. melo) and the sister species of cucumber, C. hystrix, have 2= 2= 24 chromosomes, implying a reduction from = 12 to = 7. To understand the underlying mechanisms, we investigated chromosome synteny among cucumber, C. hystrix and melon using integrated and complementary approaches. We identified 14 inversions and a C. hystrix lineage‐specific reciprocal inversion between C. hystrix and melon. The results reveal the location and orientation of 53 C. hystrix syntenic blocks on the seven cucumber chromosomes, and allow us to infer at least 59 chromosome rearrangement events that led to the seven cucumber chromosomes, including five fusions, four translocations, and 50 inversions. The 12 inferred chromosomes (AK1–AK12) of an ancestor similar to melon and C. hystrix had strikingly different evolutionary fates, with cucumber chromosome C1 apparently resulting from insertion of chromosome AK12 into the centromeric region of translocated AK2/AK8, cucumber chromosome C3 originating from a Robertsonian‐like translocation between AK4 and AK6, and cucumber chromosome C5 originating from fusion of AK9 and AK10. Chromosomes C2, C4 and C6 were the result of complex reshuffling of syntenic blocks from three (AK3, AK5 and AK11), three (AK5, AK7 and AK8) and five (AK2, AK3, AK5, AK8 and AK11) ancestral chromosomes, respectively, through 33 fusion, translocation and inversion events. Previous results (Huang, S., Li, R., Zhang, Z. et al., 2009Nat. Genet. 41, 1275–1281; Li, D., Cuevas, H.E., Yang, L., Li, Y., Garcia‐Mas, J., Zalapa, J., Staub, J.E., Luan, F., Reddy, U., He, X., Gong, Z., Weng, Y. 2011a, BMC Genomics, 12, 396) showing that cucumber C7 stayed largely intact during the entire evolution of Cucumis are supported. Results from this study allow a fine‐scale understanding of the mechanisms of dysploid chromosome reduction that has not been achieved previously.