Authors: Valerio Di Vittori, Elena Bitocchi, Monica Rodriguez, Saleh Alseekh, Elisa Bellucci, Laura Nanni, Tania Gioia, Stefania Marzario, Giuseppina Logozzo, Marzia Rossato, Concetta De Quattro, Maria L. Murgia, Juan José Ferreira, Ana Campa, Chunming Xu, Fabio Fiorani, Arun Sampathkumar, Anja Fröhlich, Giovanna Attene, Massimo Delledonne, Björn Usadel, Alisdair R. Fernie, Domenico Rau, Roberto Papa

Institutions:

  • Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy
  • Dipartimento di Agraria, Università degli Studi di Sassari, Via E. De Nicola, 07100 Sassari, Italy
  • Centro per la Conservazione e Valorizzazione della Biodiversità Vegetale, Università degli Studi di Sassari, SS 127bis, km 28.500 Surigheddu, 07041 Alghero, Italy
  • Max-Planck-Institute of Molecular Plant Physiology, Am Müehlenberg 1, Potsdam-Golm, 14476 Germany
  • Center of Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria
  • Scuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, viale dell’Ateneo Lucano 10, 85100 Potenza, Italy
  • Dipartimento di Biotecnologie, Università degli Studi di Verona, Cà Vignal 1, Strada Le Grazie 15, 37134 Verona, Italy
  • Plant Genetics Group, Agri-Food Research and Development Regional Service (SERIDA), 33300, Asturias, Spain
  • Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, 130024 China
  • Institute of Biosciences and Geosciences (IBG-2): Plant Sciences, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany

Publication: Biorxiv

Date: April 2020

Full paper: https://www.biorxiv.org/content/10.1101/2020.04.02.021972v1

Abstract:

In legumes, pod shattering occurs when mature pods dehisce along the sutures, and detachment of the valves promotes seed dispersal. In Phaseolus vulgaris, the major locus qPD5.1-Pv for pod indehiscence was identified recently. We developed a BC4/F4 introgression line population and narrowed the major locus down to a 22.5-kb region. Here, gene expression and a parallel histological analysis of dehiscent and indehiscent pods identified an AtMYB26 orthologue as the best candidate for loss of pod shattering, on a genomic region ~11 kb downstream of the highest associated peak. Based on mapping and expression data, we propose early and fine up-regulation of PvMYB26 in dehiscent pods. Detailed histological analysis establishes that pod indehiscence is associated to the lack of a functional abscission layer in the ventral sheath, and that the key anatomical modifications associated with pod shattering in common bean occur early during pod development. We finally propose that loss of pod shattering in legumes resulted from histological convergent evolution and that this is the result of selection at orthologous loci.