Authors: Olesya I. Sazonova, Olga Gavrichkova, Anastasia A. Ivanova, Kirill V. Petrikov, Rostislav A. Streletskii, Dmitriy A. Sarzhanov, Maria V. Korneykova, Andrey I. Novikov, Viacheslav I. Vasenev, Kristina V. Ivashchenko, Marina V. Slukovskaya, andAnna A. Vetrova

Institutions:

  • Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290 Pushchino, Russi
  • Research Institute on Terrestrial Ecosystems, National Research Council, 05010 Porano, Italy
  • Faculty of Soil Science, Laboratory of Ecological Soil Science, Lomonosov Moscow State University, 119991 Moscow, Russia
  • Agrarian and Technological Institute, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, Russia
  • Institute of North Industrial Ecology Problems Subdivision of the Federal Research Center “Kola Science Centre of Russian Academy of Science”, 184209 Apatity, Russia
  • I.V. Tananaev Institute of Chemistry and Technology of Rare Elements and Mineral Raw Materials, Kola Science Centre, Russian Academy of Sciences, 184209 Apatity, Russia
  • Soil Geography and Landscape Group, Wageningen University, 6707 Wageningen, The Netherlands
  • Laboratory of Nature-Inspired Technologies and Environmental Safety of the Arctic Region, Kola Science Centre, Russian Academy of Sciences, 184209 Apatity, Russia

Publication: Microorganisms

Date: October 2022

Link:  https://www.mdpi.com/2076-2607/10/10/1979

Abstract:

We performed a comparative study of the total bacterial communities and communities of cultivable polycyclic aromatic hydrocarbons (PAH)-degrading bacteria in different functional zones of Moscow and Murmansk that were formed under the influence of the PAH composition in road and leaf dust. The PAHs were determined by high-performance liquid chromatography (HPLC); the bacterial communities’ diversity was assessed by metabarcoding. The degraders were isolated by their direct plating on a medium with the PAHs. The PAH total quantity declined in the leaf dust from the traffic to the recreational zone. For the road dust, a negative gradient with pollution was observed for Rhodococcus and Acinetobacter degraders and for their relative abundance in the microbiome for the functional zones of Moscow. The opposite effect was observed in the Murmansk leaf dust for the Rothia and Pseudomonas degraders and in the Moscow road dust for Microbacterium. The PCA and linear regression analyses showed that the Micrococcus degraders in the dust were sensitive to anthropogenic pollution, so they can be used as a tool for monitoring anthropogenic changes in the biosphere. The data on the degraders’ and microbial communities’ diversity suggest that minor degrading strains can play a key role in PAH degradation.