Introductory course to RNA-seq data analysis.

Sequentia’s RNAseq summer course will introduce RNAseq data analysis to professionals from different backgrounds that work in basic research or applied R&D.

Directed to: biologists, biochemists, biotechnologists, bioinformaticians, engineers and other life science professionals that would like to broaden their knowledge on RNAseq data analysis.

Course structure:  60% practice –  40% theory. 


  • Introduction to Linux: from command line to software installation.
  • Introduction to R: syntax, installation of packages and plots generation.
  • Introduction to Next Generation Sequencing (Illumina).
  • Introduction to RNA-seq analysis: from experimental design to data analysis.

The practical sessions of the course will include:

  • Quality control of sequencing data.
  • Mapping on the reference genome.
  • Gene expression quantification.
  • Differential Expression Analysis.
  • Functional analysis of differentially expressed genes (using online tools).

Schedule: 1 week (3.5 hours per day) / Monday to Friday, 10 – 13:30 (CET)

Price: Contact us to learn the academic fees.

Dates and languages: 

  • Italian: 19-23 July
  • Spanish: 26-30 July
  • English: 2-6 Aug
  • Italian: 16-20 Aug
  • Spanish: 23-27 Aug
  • English: 30 Aug – 3 Sept

Format: Online (via Google Meets). 

Trainees per group: 7. 


  • Basic knowledge of biology and molecular biology.
  • Having a stable and fast internet connection.
  • No previous programming skills are required. The important basic concepts will be taught during the course.

Each participant will have access to his/her own cloud server for practice sessions.

An attendance certificate will be provided at the end of the course.

The course will not cover single cell RNA-seq nor metatranscriptomics analysis.


About the trainer: 

Riccardo has a fierce determination to contribute to science and technology, which led him to co-found Sequentia. Previously, he was awarded with a postdoctoral fellowship in epigenomics at the Gregor Mendel Institute of Molecular Plant Biology (GMI) in Austria. He holds a PhD in Biotechnology and a Biotechnological Sciences MSc and bachelor’s degree from University of Naples Federico II. Riccardo has extensive experience in chromatin remodelling, bioinformatics and has published over 40 papers in publications like Nature, Science and Cell.