Authors: Angela Salzano, Salvatore Fioriniello, Nunzia D’Onofrio, Maria Luisa Balestrieri, Riccardo Aiese Cigliano, Gianluca Neglia, Floriana Della Ragione, Giuseppe Campanile


  • Department of Veterinary Medicine and Animal Production, Federico II University, Naples, Italy
  • Institute of Genetics and Biophysics ‘A. Buzzati-Traverso’, CNR, Naples, Italy
  • Department of Precision Medicine, University of Campania Luigi Vanvitelli, Naples, Italy
  • Sequentia Biotech, SL, Carrer de València, 08009, Barcelona, Spain
  • IRCCS Istituto Neurologico Mediterraneo Neuromed, Pozzilli, Isernia, Italy

Publication: BMC

Date: March, 2023

Link: Transcriptomic profiles of the ruminal wall in Italian Mediterranean dairy buffaloes fed green forage


Green feed diet in ruminants exerts a beneficial effect on rumen metabolism and enhances the content of milk nutraceutical quality. At present, a comprehensive analysis focused on the identification of genes, and therefore, biological processes modulated by the green feed in buffalo rumen has never been reported. We performed RNA-sequencing in the rumen of buffaloes fed a total mixed ration (TMR) + the inclusion of 30% of ryegrass green feed (treated) or TMR (control), and identified differentially expressed genes (DEGs) using EdgeR and NOISeq tools.